Analytical
Support

Our robust capabilities support your projects.

Lexington’s experienced and capable team is committed to providing expert and timely analytical chemistry support. We offer high quality, affordable compendial testing according to USP/NF, EP, BP, JP, ACS and customer-specified monographs in support of raw materials, in-process analysis, and finished product release testing.

  • Quantitative Analysis
  • Purity Assessment
  • Microbial Assay
  • Structure Identification

Example

Detection of Impurities in the Preparation of Sucralose (Heartland Sweeteners)

Contaminants: sucrose, fructose, glucose, galactose, various partially halogenated sucralose derivatives

Techniques:High field 13C and 1H NMR, COSY, HMQC. Weighing samples to 100 ng accuracy, Serial dilution. Reversed-phase hplc, Normal-phase lc, (gc on polysilylated derivatives if necessary). UV diode Array, ELSD, RI detection

General Method: Unambiguous identification of purified components via spectroscopy. Development of a method that provides baseline separation of components. Gradient, solvent systems, stationary phases (amine, carbohydrate, C18, etc.). Correlation of retention times with compound identity. Co-injection to confirm separation, identity. UV diode array to establish "fingerprint". Serial dilution to establish sensitivity of method. Periodic standards monitoring machine performance . Runs in triplicate to determine error bars/standard deviation. Reports to customer, storage of samples and documentation according to glp.